Synchronous Sperm Penetration of Zona-Free Mouse Eggs in Vitro1
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چکیده
منابع مشابه
Preliminary study of buffalo sperm penetration into zona-free hamster eggs after treatment with calcium ionophore A23187.
A previous study (Takahashi & Hanada, 1984) reported the penetration of zona-free hamster eggs by ejaculated bull spermatozoa after treatment with calcium ionophore A23187 (Ca-IA) in the presence of caffeine. Based on this study, successful IVF was accomplished not only in cattle (Hanada, 1986b) but also in goats and sheep (Hanada, 1986a) using spermatozoa treated with Ca-IA. In the present stu...
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In most mammals that have been studied, the first spermatozoon which penetrates an egg stimulates the egg to undergo a reaction—the 'zona reaction'—which bars further spermatozoa from penetrating through the zona pellucida (Braden, Austin & David, 1954). The sequel to the zona reaction may be classified into two categories, according to species: species in which spermatozoa are entirely prevent...
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Sperm receptors are located in the mammalian egg extracellular coat, or zona pellucida. Mouse and hamster sperm receptor glycoproteins, mZP3 (83 x 10(3) M(r)) and hZP3 (56 x 10(3) M(r)), respectively, have very similar polypeptides (44 x 10(3) M(r); 81% identical) that are glycosylated to different extents. Purified mZP3 and hZP3 can bind to mouse sperm, prevent them from binding to eggs and in...
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The present paper evaluates the influence of various technical factors when preparing human spermatozoa to be tested for their ability to penetrate zona-free hamster ova. Higher in vitro fertilization rates were obtained after sperm selection by "swim-up" migration, 4-h incubation, sperm dilution to a concentration of 2.5 X 10(6) spermatozoa/ml, and observation of the oocytes 8 h after insemina...
متن کاملEthylene glycol-supplemented calcium-free media improve zona penetration of vitrified rat oocytes by sperm cells.
Cryopreservation of matured oocytes is a useful technique because the oocytes can be used for some assisted reproductive technologies after warming. Even though rats, like mice, have been used in various research fields including reproductive technology, information about cryopreservation of rat oocytes is limited. The objective of the present study was to improve the vitrification protocol for...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1995
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod53.2.424